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plko 1 mpck1 shrna plasmids  (Addgene inc)


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    Structured Review

    Addgene inc plko 1 mpck1 shrna plasmids
    Plko 1 Mpck1 Shrna Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+mpck1+shrna+plasmids/pLKO%2E1+(Plasmid+%238407)/pm40443016-147-3-32
    Average 96 stars, based on 1267 article reviews
    plko 1 mpck1 shrna plasmids - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    shRNA:

    Article Title: Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma.
    Article Snippet: Cells were then resuspended in PBS with 7-aminoactinomycin D (7-AAD) (559925, BD Biosciences) and analyzed using a CytoFLEX flow cytometer. .. The pLKO.1-scramble and pLKO.1-mPck1 shRNA plasmids were constructed by annealing and cloning 2 target double-stranded oligonucleotides (shRNA #1/ #2 sequences) and 1 non-targeting sequence (negative control, scramble) into the pLKO.1 vector (#8453, Addgene). .. Ferroptosis and cell viability were assessed by dissociating the co-cultured cancer cells, rinsing them with phosphate-buffered saline (PBS), and staining with BODIPY 581/591 C11 (D3861, Invitrogen) for 30min at 37◦C, protected from light.

    Construct:

    Article Title: Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma.
    Article Snippet: Cells were then resuspended in PBS with 7-aminoactinomycin D (7-AAD) (559925, BD Biosciences) and analyzed using a CytoFLEX flow cytometer. .. The pLKO.1-scramble and pLKO.1-mPck1 shRNA plasmids were constructed by annealing and cloning 2 target double-stranded oligonucleotides (shRNA #1/ #2 sequences) and 1 non-targeting sequence (negative control, scramble) into the pLKO.1 vector (#8453, Addgene). .. Ferroptosis and cell viability were assessed by dissociating the co-cultured cancer cells, rinsing them with phosphate-buffered saline (PBS), and staining with BODIPY 581/591 C11 (D3861, Invitrogen) for 30min at 37◦C, protected from light.

    Cloning:

    Article Title: Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma.
    Article Snippet: Cells were then resuspended in PBS with 7-aminoactinomycin D (7-AAD) (559925, BD Biosciences) and analyzed using a CytoFLEX flow cytometer. .. The pLKO.1-scramble and pLKO.1-mPck1 shRNA plasmids were constructed by annealing and cloning 2 target double-stranded oligonucleotides (shRNA #1/ #2 sequences) and 1 non-targeting sequence (negative control, scramble) into the pLKO.1 vector (#8453, Addgene). .. Ferroptosis and cell viability were assessed by dissociating the co-cultured cancer cells, rinsing them with phosphate-buffered saline (PBS), and staining with BODIPY 581/591 C11 (D3861, Invitrogen) for 30min at 37◦C, protected from light.

    Sequencing:

    Article Title: Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma.
    Article Snippet: Cells were then resuspended in PBS with 7-aminoactinomycin D (7-AAD) (559925, BD Biosciences) and analyzed using a CytoFLEX flow cytometer. .. The pLKO.1-scramble and pLKO.1-mPck1 shRNA plasmids were constructed by annealing and cloning 2 target double-stranded oligonucleotides (shRNA #1/ #2 sequences) and 1 non-targeting sequence (negative control, scramble) into the pLKO.1 vector (#8453, Addgene). .. Ferroptosis and cell viability were assessed by dissociating the co-cultured cancer cells, rinsing them with phosphate-buffered saline (PBS), and staining with BODIPY 581/591 C11 (D3861, Invitrogen) for 30min at 37◦C, protected from light.

    Negative Control:

    Article Title: Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma.
    Article Snippet: Cells were then resuspended in PBS with 7-aminoactinomycin D (7-AAD) (559925, BD Biosciences) and analyzed using a CytoFLEX flow cytometer. .. The pLKO.1-scramble and pLKO.1-mPck1 shRNA plasmids were constructed by annealing and cloning 2 target double-stranded oligonucleotides (shRNA #1/ #2 sequences) and 1 non-targeting sequence (negative control, scramble) into the pLKO.1 vector (#8453, Addgene). .. Ferroptosis and cell viability were assessed by dissociating the co-cultured cancer cells, rinsing them with phosphate-buffered saline (PBS), and staining with BODIPY 581/591 C11 (D3861, Invitrogen) for 30min at 37◦C, protected from light.

    Plasmid Preparation:

    Article Title: Simvastatin overcomes the pPCK1-pLDHA-SPRINGlac axis-mediated ferroptosis and chemo-immunotherapy resistance in AKT-hyperactivated intrahepatic cholangiocarcinoma.
    Article Snippet: Cells were then resuspended in PBS with 7-aminoactinomycin D (7-AAD) (559925, BD Biosciences) and analyzed using a CytoFLEX flow cytometer. .. The pLKO.1-scramble and pLKO.1-mPck1 shRNA plasmids were constructed by annealing and cloning 2 target double-stranded oligonucleotides (shRNA #1/ #2 sequences) and 1 non-targeting sequence (negative control, scramble) into the pLKO.1 vector (#8453, Addgene). .. Ferroptosis and cell viability were assessed by dissociating the co-cultured cancer cells, rinsing them with phosphate-buffered saline (PBS), and staining with BODIPY 581/591 C11 (D3861, Invitrogen) for 30min at 37◦C, protected from light.



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    plko 1 mpck1 shrna plasmids  (Addgene inc)
    96
    Addgene inc plko 1 mpck1 shrna plasmids
    Plko 1 Mpck1 Shrna Plasmids, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/plko+1+mpck1+shrna+plasmids/pLKO%2E1+(Plasmid+%238407)/pm40443016-147-3-32
    Average 96 stars, based on 1 article reviews
    plko 1 mpck1 shrna plasmids - by Bioz Stars, 2026-09
    96/100 stars
    		  Buy from Supplier

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